CEA/iRCM, 18 route du Panorama, BP6 , 92265 Fontenay-aux-Roses, FRANCE
Nuclear organization and genome stability
Our aim is to unravel the fundamental mechanisms determining the spatial and temporal behavior of damaged chromatin and to evaluate how chromatin organization in the nucleus affects the maintenance of genome integrity. More specifically we are studying the impact of chromatin structure and 3D nuclear organization on detection, signaling and repair of Double Strand Breaks (DSBs) using budding yeast as a model system. To do so we have developed genetic systems that allow i) to follow the position of DSB in the nuclear space and/or ii) to modify of the nuclear position and the chromatin status of a targeted DSB. Using these systems, our goals are to:
- Define how nuclear organization and chromatin status (euchromatin versus heterochromatin) influences the detection, processing and subsequent repair of DNA lesions.
- Develop proteomic and microscopy based genome-wide screens to decipher the molecular pathways controlling the position and dynamics of damaged chromatin in the nucleus.
Team:
- Cécile Bez (post-doc),
- Lionel Gellon (post-doc)