Advancing Epigenetics Towards Systems Biology

RNAse A Treatment of Mouse Cells (Prot 10)

Christèle Maison

Introduction

RNAse A treatment of permeabilized cells followed by immunostaining is a method which allows to show if the localization of a protein into the cell involves an RNAse-sensitive structure. This technique is highly dependent of the quality of the used RNAse A. In our lab, we used RNAse A treatment of mouse cells to demonstrate that the enrichment in HP1 (Heterochromatin Protein 1) proteins at pericentric heterochromatin depends on the presence of an RNA component.

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Christèle Maison

Almouzni Lab
UMR 218 Institute Curie - 26, rue d'Ulm - 75231 Paris Cedec 05, France

Christèle Maison