Advancing Epigenetics Towards Systems Biology

Cloning of small RNAs with 5’ phosphate and 3’ OH ends (Prot 40)

Ericka Havecker

Introduction

The following protocol describes a procedure for the purification and cloning of miRNAs and other small RNAs in the 20-30 nucleotide size range from plant tissue.

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Ericka Havecker

Baulcombe Lab (The Sainsbury Laboratory, Cambridge) , UK

image p40