Extended chromatin fibers can be used to examine the linear structure of chromatin by high-resolution immunofluorescent microscopy. Previous applications include studies of the distribution of canonical histone H3 chromatin and centromeric histone CenH3 chromatin at centromeres (Blower et al, 2002; Dunleavy et al, 2011; Sullivan & Karpen, 2004). This technique can be combined with fluorescent in situ hybridization (FISH) for specific DNA sequences e.g. alpha satellite arrays at centromeres (Blower et al, 2002; Lam et al, 2006) or with the incorporation of thymidine analogs (BrdU, EdU) to mark newly replicated DNA (Blower et al, 2002; Dunleavy et al, 2011).
Postdoctoral Fellow (Karpen Laboratory), Lawrence Berkeley National Laboratory, Department of Genome Dynamics, 1 Cyclotron Road, MS977, Berkeley, CA 94720, USA
Corresponding author: Elaine Dunleavy