Advancing Epigenetics Towards Systems Biology

Preparation of extended chromatin fibers from human tissue culture cells (Prot 55)

Elaine Dunleavy

Introduction

Extended chromatin fibers can be used to examine the linear structure of chromatin by high-resolution immunofluorescent microscopy. Previous applications include studies of the distribution of canonical histone H3 chromatin and centromeric histone CenH3 chromatin at centromeres (Blower et al, 2002; Dunleavy et al, 2011; Sullivan & Karpen, 2004). This technique can be combined with fluorescent in situ hybridization (FISH) for specific DNA sequences e.g. alpha satellite arrays at centromeres (Blower et al, 2002; Lam et al, 2006) or with the incorporation of thymidine analogs (BrdU, EdU) to mark newly replicated DNA (Blower et al, 2002; Dunleavy et al, 2011).

PDF version

Elaine Dunleavy

Postdoctoral Fellow (Karpen Laboratory), Lawrence Berkeley National Laboratory, Department of Genome Dynamics, 1 Cyclotron Road, MS977, Berkeley, CA 94720, USA

Corresponding author: Elaine Dunleavy
Email feedback to: This email address is being protected from spambots. You need JavaScript enabled to view it.

Elaine Dunleavy