Advancing Epigenetics Towards Systems Biology

RNA FISH on cultured cells in interphase (Prot 6)

Karen Ng

Introduction

Fluorescence in situ hybridization (FISH) has become a widely used method in genome and molecular genetic studies. The technique is highly versatile and has been adapted to carry out genome-wide screenings, microarray quantifications, cancer cytogenetics analysis, and RNA expression and localization studies. The study of intracellular RNA localization using RNA FISH provides insights into the in situ physical characteristics of transcription and intracellular RNA transport in individual cells. In our lab, we use RNA FISH to detect the localization of Xist RNA, a nuclear non-coding transcript that coats the entire chromosome from which it is transcribed.

The RNA FISH technique requires the generation of a labeled probe, hybridization of the probe to a fixed sample, and subsequently, detection of the labeled probe using microscopy.

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Karen Ng

Wutz Lab
Research Institute of Molecular Pathology (IMP)
Dr. Bohr-Gasse 7
A1030 Vienna, Austria

Karen Ng