The following "bisulfite genomic sequencing" protocol has been optimized for the determination of DNA cytosine methylation at transgene loci in plants. The conditions used are based on the method given by Pelissier and Wassenegger (2000).
Research Group Epigenetics
Department of Cytogenetics
Institute of Plant Genetics and Crop Plant Research
Corrensstraße 3 - 06466 Gatersleben, Germany