Advancing Epigenetics Towards Systems Biology

Plant DNA methylation analysis by "bisulfite genomic sequencing" (Prot 14)

Michael Mette

Introduction

The following "bisulfite genomic sequencing" protocol has been optimized for the determination of DNA cytosine methylation at transgene loci in plants. The conditions used are based on the method given by Pelissier and Wassenegger (2000).

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Michael Mette

Research Group Epigenetics
Department of Cytogenetics
Institute of Plant Genetics and Crop Plant Research
Corrensstraße 3 - 06466 Gatersleben, Germany

Michael Mette