Here we describe a modified 3C-qPCR protocol with four-fold increased efficiency of the 3C assay compared to the initially published protocols (Ref.1-4). Classical SybrGreen technique can then be used, instead of TaqMan probes, to quantify by quantitative PCR relevant 3C chimerical ligation products. It is optimized for nuclei preparation as starting material, coming from fresh tissues or cultured cells. In our hands, it was successfully used on human and mouse samples.
1 Institut de Génétique Moléculaire, UMR5535 CNRS University of Montpellier, France
2 Present address: Inserm UMR1103, CNRS UMR6293, F-63001 Clermont-Ferrand, France and Clermont Université, Université d'Auvergne, Laboratoire GReD, BP 10448, F-63000 Clermont-Ferrand, France.
Corresponding author: Franck Court