An ambitious EC-funded research initiative on epigenetics advancing towards systems biology 128

RNAse A Treatment of Mouse Cells (Prot 10)

Christèle Maison


RNAse A treatment of permeabilized cells followed by immunostaining is a method which allows to show if the localization of a protein into the cell involves an RNAse-sensitive structure. This technique is highly dependent of the quality of the used RNAse A. In our lab, we used RNAse A treatment of mouse cells to demonstrate that the enrichment in HP1 (Heterochromatin Protein 1) proteins at pericentric heterochromatin depends on the presence of an RNA component.

PDF version

Christèle Maison

Almouzni Lab
UMR 218 Institute Curie – 26, rue d'Ulm – 75231 Paris Cedec 05, France