Advancing Epigenetics Towards Systems Biology

Proteomics of isolated chromatin segments (PICh) on human telomeres (Prot 52)

Paulina Marzec, Jérôme Déjardin


Proteomics of isolated chromatin segments (PICh) is a powerful method of repetitive chromatin isolation that allows identifying proteins associated to specific genomic loci. Purification is based on nucleic acid probe hybridization to the DNA moieties present in formaldehyde-crosslinked chromatin. Hybridized chromatin is captured on magnetic beads, eluted and bound proteins can be identified by mass spectrometry. In order to increase the stability of the probe-chromatin interaction, Locked Nucleid Acids (LNA) containing oligos are used. LNA probes have higher melting temperature than DNA probes of the same sequence. This facilitates strong interactions with DNA and stabilizes probe invasion. Between the LNA probe and the immobilization tag there is a long spacer that reduces steric hindrance effects. Desthiobiotin is used as the immobilization tag. Desthiobiotin has weaker affinity to streptavidin (Kd~10-12 M) which allows a competitive gentle elution from streptavidin beads with regular biotin (Kd~10-14 M).

The following protocol is optimized for 109 cells per pull down (which is approximately the number of adherent cells growing on 50 cell culture dishes of 150 mm diameter or 25 dishes of mouse stem cells cultured on gelatin).

Each experiment should consist of at least two pulldowns: one using a scrambled and the other a telomere specific probe. In both conditions, chromatin should be processed in the same way and split just before adding LNA probes.

PDF version

Paulina Marzec, Jérôme Déjardin

Institute of Human Genetics. CNRS UPR1142. 141 rue de la Cardonille. 34000 MONTPELLIER, FRANCE.

Corresponding author: Paulina Marzec
Email feedback to: This email address is being protected from spambots. You need JavaScript enabled to view it.

Paulina Marzec, Jérôme Déjardin
Wed, Oct 16th 2019- Fri, Oct 18th 2019

The Asia Epigenome Meeting is an annual event that rotates in South Korea, Japan, China, Taiwan, Singapore and India. It is to engage world leading scientists and outstanding Asia researchers to promo...

Wed, Nov 20th 2019- Sat, Nov 23rd 2019

Metabolism and Epigenetics are intricately linked, playing a key role in development, cancer, immune signaling and aging. This symposium brings together world-leading researchers exploring this nexus....


EpiGeneSys Final
Meeting in Paris

Thur. 11 February 2016 - Sat. 13 February 2016

More than 280 scientists attended the fifth Annual Meeting of EpiGeneSys. The conference kicked off with a talk by coordinator Geneviève Almouzni, Director of the Research Center at the Institut Curie, highlighting the achievements of the network over more than five years...

Maison des océans - Paris Read more


The Non-Coding Genome ...

December 3-4 th, 2015

The last training workshop of the EpiGeneSys network

Hotel Mediterraneo - Rome, Italy Read more

Paris / TriRhena Chromatin Club

July 9th, 2015

...exciting talks and network with members of the Chromatin community!

... An EpiGeneSys TAB workshop

June 11st-12nd , 2015

... learn about current approaches to single cell epigenetics and to meet up and network with...

Montpellier, FranceRead more

Latest publications


The Histone Acetyltransferase Mst2 Protects Active Chromatin from Epigenetic Silencing by Acetylating the Ubiquitin Ligase Brl1.

Read more

Proliferation Drives Aging-Related Functional Decline in a Subpopulation of the Hematopoietic Stem Cell Compartment.

Read more

The impact of rare and low-frequency genetic variants in common disease.

Read more