Advancing Epigenetics Towards Systems Biology

Postdoc Position: "Structural Analyses of Genome Maintenance Proteins"

Institute of Molecular Biology gGmbH (IMB)

Description

The Institute of Molecular Biology (IMB) is a centre for basic research on the campus of Mainz University, Germany. We are generously funded by the Boehringer Ingelheim Foundation and the state of Rhineland-Palatinate. Our research focuses on the biology of the cell nucleus, ranging from the molecular to the organismic level. IMB researchers are supported by strong core facilities (www.imb.de/core-facilities) that offer state-of-the-art services in bioinformatics, cytometry, genomics, histology and microscopy, proteomics, and protein production. IMB’s working language is English.

In this competitive call, we are recruiting talented and ambitious scientists worldwide. At IMB, you will work on cutting-edge research projects in a highly international and interdisciplinary environment. IMB’s Postdoc Programme (IPPro) is specifically designed to meet the needs of postdocs and to equip them for a successful career in research. We offer advanced training in scientific techniques and professional skills, provide mentorship and networking opportunities, actively support your career development, and assist you with raising funds for yourself and for your projects. This will ensure that you can work efficiently and are prepared for the next step in your career.

In our 2018/19 call, we offer a postdoc positions in the lab of Prof. Eva Wolf focussing on "Structural Analyses of Genome Maintenance Proteins":

Scientific background:

The eukaryotic DNA replication fork is an important crossover point which links DNA replication, cell cycle and genome stability to circadian clocks. We are particularly interested in 3D structures of protein complexes involved in genome maintenance pathways and their functional interconnection to the circadian clock. Our technical focus lies on 3D structure determination techniques such as X-ray Crystallography and Single Particle Cryo Electron Microscopy (Cryo-EM) supported by biochemical and biophysical analyses of protein interactions. We recently published a medium (17 Å) resolution Cryo-EM structure of a mammalian TIMELESS-Tipin-RPA complex (Witosch et al., 2014). Thereby we provided the first 3D-structure of nearly full-length TIMELESS, a multitasking protein involved in various genome maintenance pathways as well as in circadian regulation.


The project:

This project aims at high resolution 3D structures of TIMELESS complexes with replication fork associated proteins and their subsequent biological validation, e.g. in mutational studies. It will involve a wide range of techniques including recombinant protein expression (E.coli, insect cells), protein purification, X-ray crystallography and Cryo-EM. Diverse biochemical and biophysical methods to study interactions and activities of the purified proteins will complement the 3D structural investigations. Our 3D structures and mechanistic in vitro studies will be functionally validated in cell-based DNA replication-, checkpoint signaling- and DNA repair assays within local collaborations.


Starting date: 1 May 2019 or later

Prior to applying, please see www.imb.de/postdocs for details on the projects offered. To apply, please send a single PDF, containing your cover letter, CV, and a concise description of your research interests and future research plans to This email address is being protected from spambots. You need JavaScript enabled to view it.. Please also arrange for two letters of recommendation to be sent to This email address is being protected from spambots. You need JavaScript enabled to view it.. IMB is an equal opportunity employer.

The deadline for applications is 29 January 2019. Interviews will take place in Mainz on 2-3 April 2019.

Declaration of consent and data protection:

By sending us your application, you consent to us saving your personal data in order to carry out the selection process. You can find more information on data protection and retention periods at www.imb.de/jobs/datenschutzerklaerung-data-protection.

Qualifications

- PhD in a relevant field
- Solid practical experience in
- Recombinant protein expression (insect cells, bacteria)
- Protein purification, in particular of protein complexes
- Cryo-EM and/or X-Ray crystallography
- Additional experience in biochemical and biophysical analyses of protein interactions is a plus
- Fluent in English (written and spoken)

Application details

Prior to applying, please see www.imb.de/postdocs for details on the projects offered. To apply, please send a single PDF, containing your cover letter, CV, and a concise description of your research interests and future research plans to This email address is being protected from spambots. You need JavaScript enabled to view it.. Please also arrange for two letters of recommendation to be sent to Dr Daniela Happel - This email address is being protected from spambots. You need JavaScript enabled to view it.

Relevant web link: https://www.imb.de/postdocs

Deadline for applications: 29th January 2019