Advancing Epigenetics Towards Systems Biology

Sigurd Braun

Department of Physiological Chemistry in the Adolf Butenandt Institute, at the Ludwig Maximilians University of Munich

Regulatory networks of heterochromatin formation

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sigurd braun

Our lab is interested in dissecting the regulatory pathways and networks that control heterochromatin in the model system fission yeast (S. pombe). To identify systematically new epigenetic regulators, extensive genetic screens will be performed that account for two major challenges: redundancy in functional pathways and difficulties in detection of weak phenotypes. The isolated regulators will then be mapped to a comprehensive network by employing functional genetics. This quantitative approach is based on the epistasis mini-array profiling (E-MAP) method, which allows dissecting epistatic and parallel pathways and assigning them to functional groups. Finally, to address the dynamic changes that heterochromatin undergoes, we are developing a fluorescence-based silencing reporter systems, in which reporter gene expression can be directly measured at high precision and the single-cell level. This approach will gather information with high spatial and temporal resolution and may provide sufficiently dense quantitative data to perform stochastic analysis of heterochromatin dynamics.

People involved:
Dr. Ramón Ramos Barrales, Postdoc

Latest publications

The Histone Acetyltransferase Mst2 Protects Active Chromatin from Epigenetic Silencing by Acetylating the Ubiquitin Ligase Brl1.

28648780 - 2017-06-27
Mol Cell 2017 Jun 14;

Flury V, Georgescu PR, Iesmantavicius V, Shimada Y, Kuzdere T, Braun S, Bühler M

Chromatin binding and silencing: Two roles of the same protein Lem2.

28357352 - 2017-03-31
Microb Cell 2016 Apr 4;3(4):185-188

Barrales RR, Braun S

Sensitive and Quantitative Three-Color Protein Imaging in Fission Yeast Using Spectrally Diverse, Recoded Fluorescent Proteins with Experimentally-Characterized In Vivo Maturation Kinetics.

27479698 - 2016-08-02
PLoS One 2016;11(8):e0159292

Al-Sady B, Greenstein RA, El-Samad HJ, Braun S, Madhani HD

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