Cancer Institute, University College London, London, UK
Higher order nuclear organization in mammalian stem cells
We are interested in identifying the DNA sequences, chromatin proteins and RNA species which contribute to nuclear organization. To this end, we are using unbiased genome-wide molecular methods such as HiC to describe the higher order organization of the nucleus in mammalian stem cells. We focus our current efforts on cohesin proteins. The evolutionarily conserved cohesin complex has fundamental roles in chromosome biology. These include sister chromatid cohesion, post-replicative DNA repair and the control of transcription. Specifically, we aim to address the mechanisms used by cohesin proteins to mediate their effects on gene expression. It is increasingly clear that cohesin proteins act as key players in mediating chromatin interactions between distal cis-regulatory elements that are essential for appropriate gene expression. We use mammalian post-mitotic cells derived from the neural lineage to investigate genome-wide cohesin-based chromatin organization and gene expression under conditions where cohesins are not essential for cell division.
Residing in the physical heart of the cell, the nucleus has now fully shed its once one-dimensional reputation as the repository for genetic information and steady supplier of messages to the cytoplasm. This sea change…
An open call for bilateral Franco-German projects in human epigenomics from the ANR-France has been announced! The deadline to submit a "declaration of intention" is March 29th, 2013. Click here for the announcement (in French).
Edith Heard, named a Chair of the Collège de France in Epigenetics and Cellular Memory will be giving weekly lectures starting in February that, in the tradition of this great institution, are free for anyone to attend. Lectures (in French) are from 16-17:30…
Watch the Nobel Prize winner, Sir John Gurdon, speak about winning the prize and about his revolutionary work on nuclear reprogramming.