Unit of Virus Host-Cell Interactions, European Molecular Biology Laboratory, Grenoble Outstation, Grenoble, France
Structure of Chromatin Modifying Machinery
We use X-ray crystallography as a central technique to obtain structural information on protein and protein-RNA complexes involved in RNA metabolism, translation, virus replication and epigenetics. Within epigenetics we focus on structural characterization of the Dosage Compensation (MSL) and Non-specific lethal (NSL) complexes. The MSL complex is a key player of the dosage compensation mechanism that balances expression levels of X-chromosomal genes between male and female. In Drosophila males it binds along the X-chromosome and acetylates H4 histones ultimately resulting in a two-fold up-regulation of most of the X-linked genes. The NSL complex is a newly identified major transcriptional regulator which also contains the histone acetyl-transferase MOF. We determine crystal structures of individual subunits or defined sub-complexes. The structural information is then used in in vitro and in vivo functional assays (in collaboration with the group of Dr. Asifa Akhtar) that help us understand how these large chromatin modifying machines function.
Residing in the physical heart of the cell, the nucleus has now fully shed its once one-dimensional reputation as the repository for genetic information and steady supplier of messages to the cytoplasm. This sea change…
An open call for bilateral Franco-German projects in human epigenomics from the ANR-France has been announced! The deadline to submit a "declaration of intention" is March 29th, 2013. Click here for the announcement (in French).
Edith Heard, named a Chair of the Collège de France in Epigenetics and Cellular Memory will be giving weekly lectures starting in February that, in the tradition of this great institution, are free for anyone to attend. Lectures (in French) are from 16-17:30…
Watch the Nobel Prize winner, Sir John Gurdon, speak about winning the prize and about his revolutionary work on nuclear reprogramming.